Nuclear magnetic resonance line-shape analysis of fluorine-19-labeled phospholipids

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Abstract
The application of a 19F probe to the problem of motions present in the hydrophobic region of phospholipid dispersions and biological membranes was extended to the study of phospholipids labeled with 19F in the 8 position and with 2H in the 2, 7, and 9 positions of the 2-acyl chain. 1-Myristoyl-2-(8,8-difluoro[2,2,7,7,9,9-2H6]myristoyl)-sn-glycero-3-phosphocholine and its nondeuterated analog were investigated by 19F NMR at 282.4 MHz. Spectra obtained from macroscopically oriented bilayers exhibit Pake doublets from which order parameters can be obtained. The spectra obtained from nonoriented liposomes of the phospholipids can be explained in a satisfactory manner as a random superposition of doublets broadened by heteronuclear magnetic dipole-dipole interactions. From an analysis of the data, several conclusions about the motional state of the hydrocarbon chains in the liquid-crystalline phase can be drawn. Appropriate 19F probes in the acyl chains of phospholipids can be used to investigate the structure and dynamics of model and biological membranes.

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