Evaluation of the AutoMicrobic system for identification of glucose-nonfermenting gram-negative rods

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Abstract
With the Enterobacteriaceae Plus Biochemical Card, the AutoMicrobic system is purported to identify members of the family Enterobacteriaceae and 7 spp. of glucose-nonfermenting gram-negative rods: Pseudomonas aeruginosa, P. fluorescens, P. putida, P. maltophilia, P. cepacia and Acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). The latter capacity was examined in this investigation. Of 410 glucose-nonfermenting rods included in the identification profile, the AutoMicrobic system correctly identified 366 (89.3%). Of 62 glucose-nonfermenting organisms not included in the identification profile, 41 (66%) were correctly reported as unidentified organisms. The usefulness of the AutoMicrobic system-generated identification probability in establishing criteria for acceptance of identification is discussed.