A chromosome-specific microdissected library increases marker density on bovine chromosome 1.
Open Access
- 1 January 1997
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 7 (1) , 76-80
- https://doi.org/10.1101/gr.7.1.76
Abstract
Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.Keywords
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