Hydroxylation of p‐Coumaric Acid by Illuminated Chloroplasts
Open Access
- 1 July 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 55 (2) , 355-360
- https://doi.org/10.1111/j.1432-1033.1975.tb02169.x
Abstract
1 Chloroplasts isolated from leaves of spinach-beet (Beta vulgaris L. ssp. vulgaris) do not catalyse the hydroxylation of p-coumaric acid in the dark unless a reductant (such as ascorbate, NADH or NADPH) is added. Superoxide dismutase has no effect on this reaction. 2 Illuminated chloroplasts catalyse the hydroxylation in the absence of added reductant. This reaction is completely inhibited by superoxide dismutase, but catalase has little effect. 3 Both hydroxylation in the light and hydroxylation in the dark in the presence of reductants are inhibited by diethyldithiocarbamate, EDTA, cyanide and 2-mercaptoethanol. 4 It is proposed that O2.− generated by illuminated chloroplasts is involved in the provision of a reductant to the enzyme phenolase.Keywords
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