Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene
Top Cited Papers
- 1 June 2000
- journal article
- research article
- Published by Springer Nature in Nature Genetics
- Vol. 25 (2) , 144-146
- https://doi.org/10.1038/75985
Abstract
We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization1,2,3,4 (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D (ref. 6), mapped to the other.Keywords
This publication has 10 references indexed in Scilit:
- Genome-wide analysis of DNA copy-number changes using cDNA microarraysNature Genetics, 1999
- Klinefelter's syndrome as a model of anomalous cerebral laterality: Testing gene dosage in the X chromosome pseudoautosomal region using a DNA microarrayDevelopmental Genetics, 1998
- High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarraysNature Genetics, 1998
- Positional cloning of ZNF 217 and NABC 1 : Genes amplified at 20q13.2 and overexpressed in breast carcinomaProceedings of the National Academy of Sciences, 1998
- Matrix-based comparative genomic hybridization: Biochips to screen for genomic imbalancesGenes, Chromosomes and Cancer, 1997
- A bacterial artificial chromosome-based framework contig map of human chromosome 22q.Proceedings of the National Academy of Sciences, 1996
- A Novel Angiogenic Molecule Produced at the Time of Chondrocyte Hypertrophy during Endochondral Bone FormationDevelopmental Biology, 1996
- Chapter 15 Fluorescence in Situ Hybridization for the Detection of DNA and RNAPublished by Elsevier ,1995
- Preparation and screening of an arrayed human genomic library generated with the P1 cloning system.Proceedings of the National Academy of Sciences, 1994
- Mapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization.Genetics, 1993