Isolation and characterization of the minimal fragment required for autonomous replication (“Basic replicon”) of a copy mutant (pKN102) of the antibiotic resistance factor R1
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 162 (1) , 51-57
- https://doi.org/10.1007/bf00333850
Abstract
The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the “basic replicon” is a DNA segment not larger than 1.8 kb, which carries the origin of replication and the genetic information for at least two proteins. Protein F (MW=11.000 dalton) seems to be synthesed in larger amounts in minicells of E. coli than protein C (20.000 dalton). Plasmids containing this isolated replicon of R1 are completely compatible with the parental plasmid R1drd-19.This publication has 22 references indexed in Scilit:
- Site specific recombination involved in the generation of small plasmidsMolecular Genetics and Genomics, 1978
- Replication of the mini-R1 plasmid Rsc11 and Rsc11 hybrid plasmidsMolecular Genetics and Genomics, 1977
- Characterization of small ampicillin resistance plasmids (Rsc) originating from the mutant antibiotic resistance factor R1drd-19B2Molecular Genetics and Genomics, 1977
- Transposition and insertion of intact, deleted and enlarged ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into transfer factorsMolecular Genetics and Genomics, 1977
- Properties of hybrid plasmids, consisting of parts of the mini-R1 factorRsc11 andColE1Molecular Genetics and Genomics, 1977
- Dissociation and Recombination of Fragments with Defined Functions of the Antibiotic Resistance Factor R1Published by Springer Nature ,1977
- Two origins of replication in composite R plasmid DNANature, 1976
- Cloning, isolation, and characterization of replication regions of complex plasmid genomes.Proceedings of the National Academy of Sciences, 1975
- Construction of Biologically Functional Bacterial Plasmids In VitroProceedings of the National Academy of Sciences, 1973
- Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: II. Structure of drug resistance (R) factors and f factorsJournal of Molecular Biology, 1973