Measurement of renal function in the rat with single injection clearances

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Abstract
A method for measuring renal function in the rat is described. Anesthetized rats were injected intravenously with Ortho -iodohippurate 1^5 and the radioactivity was continuously monitored over the head for 60 min. Two blood samples were drawn from the tail or the retro-orbital plexis of the eye at 10 min,and at 60 min. after the injection. The renal clearance (ERPF) was calculated from the components of the head curve after calibration with the measured plasma radioactivity at 10 and 60 min. This method gave results which were not statistically different from PAH [para-aminohippurate] clearances performed in the same animals. The average clearance in 14 normal rats was 2.11 [plus or minus] 0.15 (SE) ml/min. per 100 g. The method is reproducible and can be performed serially.