Efficient translation and polyribosome binding of 125I‐labelled rabbit globin messenger ribonucleoprotein
- 7 March 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 153 (1) , 119-124
- https://doi.org/10.1016/0014-5793(83)80130-6
Abstract
Rabbit polyribosomal globin messenger ribonucleoprotein (mRNP) was labelled under mild conditions, using 125I and Iodogen, in the protein moiety so that the fate of mRNA-associated proteins could be followed during translation. 125I-mRNP was shown to retain functional activity in the nuclease-treated reticulocyte lysate translation system under optimal labelling conditions. Polyribosome binding of 125I-mRNP and its sensitivity to cycloheximide indicated a functional- and translation-dependent binding of mRNP proteins. The results constitute a successful and direct approach to the study of mRNA-associated proteins in translational controlKeywords
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