Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury
Top Cited Papers
- 11 December 2001
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (26) , 15306-15311
- https://doi.org/10.1073/pnas.251466698
Abstract
Globins are oxygen-binding heme proteins present in bacteria, protists, fungi, plants, and animals. Their functions have diverged widely in evolution, and include binding, transport, scavenging, detoxification, and sensing of gases like oxygen, nitric oxide, and carbon monoxide. Neuroglobin (Ngb) is a recently discovered monomeric globin with high affinity for oxygen and preferential localization to vertebrate brain. No function for Ngb is known, but its affinity for oxygen and its expression in cerebral neurons suggest a role in neuronal responses to hypoxia or ischemia. Here we report that Ngb expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and that neuronal survival after hypoxia is reduced by inhibiting Ngb expression with an antisense oligodeoxynucleotide and enhanced by Ngb overexpression. Both induction of Ngb and its protective effect show specificity for hypoxia over other stressors. We conclude that hypoxia-inducible Ngb expression helps promote neuronal survival from hypoxic-ischemic insults.Keywords
This publication has 31 references indexed in Scilit:
- Biochemical Characterization and Ligand Binding Properties of Neuroglobin, a Novel Member of the Globin FamilyJournal of Biological Chemistry, 2001
- The Heme Environment of Mouse NeuroglobinJournal of Biological Chemistry, 2001
- Human Neuroglobin, a Hexacoordinate Hemoglobin That Reversibly Binds OxygenPublished by Elsevier ,2001
- Cellular Mechanism of Oxygen SensingAnnual Review of Physiology, 2001
- Multiple Molecular Penumbras after Focal Cerebral IschemiaJournal of Cerebral Blood Flow & Metabolism, 2000
- In Situ Detection of Neuronal DNA Strand Breaks Using the Klenow Fragment of DNA Polymerase I Reveals Different Mechanisims of Neuron Death After Global Cerebral IschemiaJournal of Neurochemistry, 1999
- Problems and possible solutions in finding an unrelated bone marrow donor. Results of consecutive searches for 240 Dutch patientsBone Marrow Transplantation, 1997
- Hypoxia Response Elements in the Aldolase A, Enolase 1, and Lactate Dehydrogenase A Gene Promoters Contain Essential Binding Sites for Hypoxia-inducible Factor 1Journal of Biological Chemistry, 1996
- Globin and Globin Gene Structure of the Nerve Myoglobin of Aphrodite aculeataJournal of Biological Chemistry, 1996
- Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 1989