Hemmung der Lactatdehydrogenase durch Adenosinphosphate

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Abstract
Lactate dehydrogenase from human tissues is competitively inhibited by ATP, ADP and AMP, which compete with NADH. A 5 [image] -phosphate ester of adenosine is necessary for the inhibitory action, since adenosine itself causes only slight inhibition. Under the conditions studied, only the reaction pyruvate + NADH + H[image][forward arrow] lactate + NAD[image] is affected, and not the reverse reaction. The Michaelis constants Km(NADH) for the isoenzymes I and V of lactate dehydrogenase and the inhibitor constant Ki for the inhibition by adenosine phosphates are reported.