Revision of Estimates of the Molecular Weights of Tobravirus Coat Proteins
- 1 January 1975
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 5 (6) , 313-318
- https://doi.org/10.1159/000149929
Abstract
The MW''s of proteins from several tobacco rattle viruses (or tobraviruses) were determined by electrophoresis with marker proteins in acrylamide gels of different strengths. Plotting KR, a measure of the effect of varying the gel strength, against MW gave a value of 23,000 .+-. 2,800 for tobacco rattle virus (TRV) strain CAM and similar values for other strains of TRV and pea early-browning virus. A value of 21,700 .+-. 700 was obtained for TRV strain PRN by sedimentation to equilibrium in 6 M guanidine hydrochloride. These estimates are similar to those made by a statistical reappraisal of published data on amino acid composition and a value of about 22,000 is proposed for TRV coat proteins.This publication has 1 reference indexed in Scilit:
- Electron microscopic observations on the substructure of tobacco rattle virusJournal of Molecular Biology, 1966