In vitro Studies of Hyperacute Rejection of Xenografts

Abstract
Pig leukocytes grown in culture using dog and human sera were used to study the mechanisms of hyperacute rejection of xenografts. A strong cytotoxic complement-dependent antibody in dog serum produced lysis of pig leukocytes, red cells and platelets within minutes. Pig leukocytes grew well in complement inactivated dog serum but the viable cell count was reduced and red cells were agglutinated. Human and rabbit serum also contained a complement-dependent cytotoxic antibody.

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