An Agarose Plate Method for Detecting Alloantisera to Coagulant Factor IX and Factor IX Antigen

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Abstract
An agarose plate method for detecting human alloantibodies (inhibitors) to coagulant factor IX was developed. The assays was based on the ability of such antisera to inhibit coagulation of a mixture of hemophilia B plasma, normal plasma and platelet substitute in an agarose matrix. The agarose plate method was also adopted to measure levels of FIX antigen (IX:Ag) in plasma. Using this technique, 12 of 17 obligate carriers of hemophilia B demonstrated reduced levels of IX:Ag. Of the 5 carriers with normal IX:Ag levels, 3 were members of kindred in which affected individuals had normal or near normal levels of IX:Ag.