Correction for Plasma Trapped in the Red Cell Column of the Hematocrit

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Abstract
1. In the hematocrit, the percentage of the red cell column occupied by trapped plasma increases directly with the packed cell volume; this is due to a corresponding reduction of the effective radius of centrifugation. 2. When P.C.V. is determined in Wintrobe hematocrit tubes spun at 3000 r.p.m. in a centrifuge of radius 15 cm., the variation in plasma trapping between one sample and another is much smaller after 55 minutes centrifugation than after 30 minutes. If a 55 minute period is used, variation is so small, even when pathologic bloods are tested, that it is satisfactory to use a calibration curve. Such a curve is presented.
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