Imaging receptor microdomains on leukocyte subsets in live mice
- 25 February 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (3) , 219-222
- https://doi.org/10.1038/nmeth1018
Abstract
We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.Keywords
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