Differential Response of Rat Tooth Germs to a Murine Sarcoma Virus in Cell Culture and Syngeneic Transplants2

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Abstract
Tooth germs from 16-, 17-, and 18-day Wistar/Furth (W/Fu) rat embryos were grownin cell or organ cultures and in synqeneic newborn rats to determine oncogenic responses to the Kirsten murine sarcoma virus. Isolated, dispersed epithelium did not respond to virus infection, whereas mesenchymal cells developed typical foci of morphologically transformed cells. Control and virus-infected tooth germs of all stages grew well in agar medium for up to 7 days. Characteristic cytodifferentiation and histodifferentiation were observed. Noninfected tooth germs differentiated into advanced structures with calcification of the extracellular matrix after subcutaneous transplantation into W/Fu neonates for 30 days. Virus-infected tooth germs had altered odontoblastic precursor cells isolated within the mesenchyme after 14 days' transplantation. The characteristic phenotype of odontoblasts and the orderly deposition of extracellular matrix were lost. Amelogenesis opposite altered mesenchymal areas was retarded or arrested. Long-term syngeneic transplantation (>350 days) of virus-infected tooth germs resulted in the development of transplantable sarcomas. Notumo~sdeveloped in long-term transplants of noninfected rat tooth germs.

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