Molecular Mechanism of Polyploidization and Binucleate Formation of the Hepatocyte
- 1 January 1977
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 2 (3) , 261-265
- https://doi.org/10.1247/csf.2.261
Abstract
A cross-linking agent (actinomycin D or mitomycin C) was administered to newborn rats with liver cells actively proliferating in the diploid state, to test the recently proposed hypothesis that polyploidization and binucleate formation become manifest, during abortive cell proliferation in the nucleus with cross-links between double strands of DNA. The polyploidization of hepatocyte nuclei was analyzed by Feulgen cytofluorometry with smear preparations, and binucleate formation was determined on section preparations. The number of polyploid and binuclear cells increased in rat liver treated with a cross-linking agent in comparison with the control. This finding supports the cross-linkage hypothesis.Keywords
This publication has 2 references indexed in Scilit:
- Polyploïdie somatique dans le foie de ratExperimental Cell Research, 1966
- The occurrence and formation of binucleate and multinucleate cells and polyploid nuclei in the mouse liverJournal of Anatomy, 1948