Biochemical Characteristics of Membrane Fractions Isolated from Maize (Zea mays L.) Roots

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Abstract
Membrane fractions have been isolated from maize ( Zea mays L.) roots by discontinuous density gradient centrifugation and phase separation methods. A number of approaches were tried with the aim of identifying specific membrane types, especially the plasma membrane. These included the use of enzymic markers, determination of glucose and leucine incorporation, separation of membrane proteins by SDS-PAGE, and attempts to identify the plasma membrane fraction by cell surface labelling. The results are discussed in relation to the usefulness of membrane markers and the difficulties of isolating surface membranes from higher plant tissues.

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