Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster
- 1 June 1993
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (12) , 3887-3892
- https://doi.org/10.1128/jb.175.12.3887-3892.1993
Abstract
A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.Keywords
This publication has 36 references indexed in Scilit:
- Characterization of a novel regulatory gene governing the expression of a polyketide synthase gene in Streptomyces ambofaciensMolecular Microbiology, 1992
- The use of a rare codon specifically during development?Molecular Microbiology, 1991
- The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of streptomycesCell, 1991
- Transcript mapping using [35S]DNA probes, trichloroacetate solvent and dideoxy sequencing ladders: a rapid method for identification of transcriptional start pointsGene, 1988
- Cloning and analysis of the promoter region of the erythromycin-resistance gene (ermE) of Streptomyces erythraeusGene, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Identity of cosynthetic factor I of Streptomyces aureofaciens and fragment FO from coenzyme F420 of Methanobacterium speciesJournal of the American Chemical Society, 1982
- Adrenergic and Cholinergic Receptors of Cerebral MicrovesselsJournal of Cerebral Blood Flow & Metabolism, 1981
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976