Furunculosis Injection Model for Drug Efficacy Testing of Seawater-Adapted Atlantic Salmon

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Abstract
We describe an injection model for creating furunculosis, caused by Aeromonas salmonicida, in seawater-adapted Atlantic salmon Salmo salar and for testing the efficacy of chemotherapeutants. An injection model was most appropriate for chemotherapeutant studies. Isolate selection was based on determination of drug resistance and the pathogenicity of the isolate, both of which can vary widely. Fish were handled without anesthesia with minimal handling stress; their feeding behavior was normal. Preliminary challenge trials demonstrated highly variable pathogenicity for different isolates of A. salmonicida. In the main four-dose challenge of 30, 90, 300, and 900 bacteria per fish, cumulative 16-d mortality rates ranged from 28% to 38%. Onset of deaths was earlier for the two higher doses, and these doses resulted in greater cumulative mortality than did the lower two doses. Power analysis of the data showed that with a five-replicate study, a 12.1 % difference in cumulative mortality rate between the...

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