Effect of Certain Divalent Cations on the Dye-Binding Capacities of Casein and Milk

Abstract
The divalent cations of Ca, Mg, Mn and Zn increased the orange-G dye binding capacities of casein and milk. The increase in milk occurred at lower levels of cation than in casein due to the presence of significant amounts of cations in untreated milk. The intramolecular H bonds on the protein between cationic N and carboxyl or hydroxyl groups may be severed making additional cationic N centers available for the attacking dye anion.

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