The lack of genetic information on buffalograss [Buchloë dactyloides (Nutt.) Engelm.] may be due to the high ploidy level (2n = 40 and 2n = 60) of all reported U.S. germplasm. This study was conducted to confirm the existence of a Mexican diploid (2n = 20) race and to initiate genetic analysis in buffalo‐grass by examining male‐to‐female sex ratios and inheritance of anther and stigma color. Cytological investigation confirmed the existence of the diploid race (2n = 20). Sex ratios were 1:1 at the population level, but were significantly (P < 0.01) male biased for particular parents. A polygenic sex determining mechanism was hypothesized. Anther color was either red or blue and stigma color was either purple or clear (absence of pigmentation), and were found to be readily observable and consistently expressed. Blue anther color was controlled by a single recessive allele that was assigned the symbol b. Clear stigmas were inherited as a bifactorial double recessive trait