Multichannel homogeneous immunoassay for detection of 2,4,6‐trinitrotoluene (TNT) using a microfabricated capillary array electrophoresis chip
- 17 June 2004
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 25 (12) , 1895-1900
- https://doi.org/10.1002/elps.200305818
Abstract
A high-throughput homogeneous immunoassay for the sensitive detection of 2,4,6-trinitrotoluene (TNT) has been developed using radial capillary array electrophoresis microdevices. Samples consisting of equilibrium mixtures of anti-TNT antibody (Ab), fluorescein-labeled TNT, and various concentrations of unlabeled TNT were electrokinetically injected into 48 channels of a radial capillary array electrophoresis microchannel plate. The rapid electrophoretic separation allows us to analyze the equilibrium ratio formed by the competition between the labeled and the unlabeled TNT for Ab binding. The simultaneous parallel TNT separations facilitate determination of a calibration curve for the TNT assay, which has high sensitivity (LOD, 1 ng/mL) and a wide dynamic range (1–300 ng/mL).Keywords
This publication has 29 references indexed in Scilit:
- Miniaturization of a Homogeneous Fluorescence Immunoassay Based on Energy Transfer Using Nanotiter Plates as High-Density Sample CarriersAnalytical Chemistry, 2001
- Micromosaic ImmunoassaysAnalytical Chemistry, 2000
- Miniaturized FRET assays and microfluidics: key components for ultra-high-throughput screeningDrug Discovery Today, 1999
- Monoclonal antibody binding affinity determined by microchip‐based capillary electrophoresisElectrophoresis, 1998
- Microchip-Based Capillary Electrophoresis for Immunoassays: Analysis of Monoclonal Antibodies and TheophyllineAnalytical Chemistry, 1997
- Micromachinng chemical and biochemical analysis and reaction systems on glass substratesSensors and Actuators B: Chemical, 1996
- Microchip Electrophoretic Immunoassay for Serum CortisolAnalytical Chemistry, 1996
- Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillariesAnalytical Chemistry, 1994
- Continuous-flow immunosensor for detection of explosivesAnalytical Chemistry, 1993
- Capillary array electrophoresis using laser-excited confocal fluorescence detectionAnalytical Chemistry, 1992