A Chromosome Integration System for Stable Gene Transfer into Thermus flavus
- 1 March 1995
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 13 (3) , 271-275
- https://doi.org/10.1038/nbt0395-271
Abstract
We have developed a chromosomal integration system for gene transfer into the extreme thermophile Thermus flavus. The system relies on integration at the site of leuB (3-isopropylmalate dehydrogenase) which was cloned from T. flavus. The leuB gene was insertionally inactivated in vitro with a thermostable kanamycin-resistance gene and transformed in single-copy into the chromosome of T. flavus on a plasmid vector. Gene replacement strains required leucine for growth, were stably kanamycin-resistant and could grow hi the presence of kanamycin at temperatures up to 55°C.Keywords
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