Ester Synthesis by NAD+-dependent Dehydrogenation of Hemiacetal: Production of Methyl Formate by Cells of Methylotrophic Yeasts
- 1 January 1997
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 61 (8) , 1391-1393
- https://doi.org/10.1271/bbb.61.1391
Abstract
A water-soluble ester, methyl formate, was detected as a metabolite in the culture medium of methylotrophic yeasts. Methyl formate synthase, which catalyses NAD+-dependent dehydrogenation of the hemiacetal adduct of methanol and formaldehyde, catalyses the ester synthesis. The enzyme activity was induced on a methanol medium and was increased further by the addition of formaldehyde. In the reaction system using intact cells of Pichia methanolica AKU 4262, 135 mm (8.1 g/liter) methyl formate was produced from 2 m methanol. This is a new biological process for ester synthesis that couples spontaneous formation of hemiacetal and alcohol dehydrogenase.Keywords
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