Mammary Tumor Virus (MTV) Infection of CBA/Bin Mice Involving Production of Antibodies to MTV

Publisher Website
Google Scholar
Abstract
With acetone-fixed, frozen-cut slices of syngeneically grafted CBA/Bln murine mammary tumors extremely rich in mammary tumor virus (MTV) A and B particles, spontaneously occurring antibodies could be detected in 43 of 46 MTV-infected mice by means of the indirect immunofluorescent technique. These antibodies were found in male and female MTV-infected CBA/Bln and XVII/Bln mice but not in MTV-free mice of both strains. The antibodies could be absorbed totally by MTV-containing milks of the two strains, whereas MTV-free milks of both strains failed to do so. In correspondence to the specificity of precipitating antibodies detectable in a limited number of infected mice, which are directed against the B particles or an antigen of the outer viral coat, the resulting fluorescence was restricted to the intercellular spaces and glandular lumina of the tumor tissue. The results show that no trace of any true immunologic tolerance to this particular MTV antigen is established in CBA/Bln mice. Furthermore, this type of antibody response seems to be the most sensitive indicator for an MTV infection.

This publication has 0 references indexed in Scilit: