Analysis of Penicillic Acid by Gas-Liquid Chromatography
Open Access
- 1 July 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 57 (4) , 861-865
- https://doi.org/10.1093/jaoac/57.4.861
Abstract
A gas-liquid chromatographic (GLC) method is described for the analysis of the mycotoxin penicillic acid in corn, dried beans, and apple juice. The samples are extracted with ethyl acetate and the penicillic acid is isolated by liquidliquid extraction, followed by elution from a 3 g silica gel column with hexane-ethyl acetate. Penicillic acid is determined by GLC of the trifluoroacetate derivative, using a 6' column containing OV-101 and an electron capture detector. Penicillic acid can be detected at the 4 μg/kg level with recoveries of added penicillic acid greater than 80%. Confirmation may be obtained by gas chromatography-mass spectrometry. Analysis of 20 commercial corn samples originating from several Midwest counties with potential mold problems showed that penicillic acid was present in 7 samples at levels ranging from 5 to 230 μg/kg. Penicillic acid was found in 5 of 20 commercial samples of dried beans at levels of from 11 to 179 μg/kg.Keywords
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