REVERSE PLAQUE-FORMATION METHOD FOR TITRATION OF NON-CYTOPATHOGENIC BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 23  (1) , 27-31
Abstract
The reverse plaque formation (RPF) method with a semi-micro plate was applied to the titration of a non-cytopathogenic (non-CP) strain of bovine viral diarrhea-mucosal disease (BVD-MD) virus. All 5 non-CP stains used in this experiment formed reverse plaques (RP) on bovine testicle cell culture under methyl cellulose overlay. RPF was inhibited by pretreatment of a non-CP virus strain with immune rabbit serum. The specificity of the RPF method was demonstrated by a linear test and Poisson distribution test. Comparative titration of commercial BVD-MD vaccines was carried out by the semi-micro RPF and tube methods. The virus titer obtained by the former was slightly higher than that obtained by the latter. The former was determined to be a method of high sensitivity for determining non-CP virus.