Endogenous 15‐deoxy‐Δ12,14‐prostaglandin J2 synthesized by adipocytes during maturation phase contributes to upregulation of fat storage
Open Access
- 29 November 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (30) , 6885-6890
- https://doi.org/10.1016/j.febslet.2006.11.049
Abstract
15‐Deoxy‐Δ12,14‐prostaglandin J2 (15d‐PGJ2) has been identified as a natural ligand for peroxisome proliferator‐activated receptor (PPAR) γ to promote adipogenesis. However, it remains elusive about the ability of PPARγ‐expressing adipocytes to produce PGJ2 series and the role in the life cycle of adipocytes. Here, we developed an enzyme‐linked immunosorbent assay specific for 15d‐PGJ2. The analysis using this method revealed the increase in the endogenous synthesis of immunoreactive 15d‐PGJ2 in cultured adipocytes during the maturation phase. Further studies using cyclooxygenase inhibitors clarified the contribution of endogeous 15d‐PGJ2 produced by mature adipocytes to upregulation of fat storage in an autocrine manner.Keywords
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