Serum fractionation on immobilized pH gradients with one‐ and two‐dimensional techniques

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Abstract
Immobilized pH gradients covering, in presence of 8 Murea, the intervals pH 4–7, pH 4–8 and pH 4–9 were applied tothe analysis of human serum. Both formulations with constant buffering power and with constant ionic strengthd were tried. Results are presented of the isoelectric focusing step and of a further fractionation of the sample by sodium dodecyl sulfate‐ polyacrylamide gel electrophoresis in a two‐dimensional by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis in a two‐dimensional system.