Purification and Characterization of a Hemagglutinin from Arachis hypogaea

Publisher Website
Google Scholar
Abstract
A. hypogaea hemagglutinin was purified by (NH4)2 SO4 fractionation and Sepharose 6 B column chromatography. The homogeneity of the purified hemagglutinin was ascertained by ultracentrifugal analysis and polyacrylamide gel electrophoresis. It has a MW of 106,500 and is a tetramer of a subunit with a MW of 27,000. The purified hemagglutinin agglutinated neuraminidase-treated human erythrocytes regardless of their ABO group type, but did not agglutinate intact erythrocytes. In hapten inhibition assays with simple sugars, the so-called Makela''s group 2 sugars, which bear the same configuration of hydroxy groups at C-3 and C-4 as D-galactopyranose, were inhibitors for this hemagglutinin. It does not contain any carbohydrate, in contrast to most phytohemagglutinins except Concanavalin A and wheat germ agglutinin.

This publication has 1 reference indexed in Scilit: