Characterization of a DNA binding protein of bacteriophage PRD1 involved in DNA replication
- 25 November 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (22) , 6553-6557
- https://doi.org/10.1093/nar/18.22.6553
Abstract
Eschehchia coli phage PRD1 protein P12, involved In PRD1 DNA replication in vivo, has been highly purified from E. coli cells harboring a gene Xll-containing plasmid. Protein P12 binds to single-stranded DNA as shown by gel retardation assays and nuclease protection experiments. Binding of protein P12 to single-stranded DNA increases about 14% the contour length of the DNA as revealed by electron microscopy. Binding to single-stranded DNA seems to be cooperative, and it is not sequence specific. Protein P12 also binds to double-stranded DNA although with an affinity 10 times lower than to single-stranded DNA. Using the in vitro phage Φ29 DNA replication system, it is shown that protein P12 stimulates the overall Φ29 DNA replication.This publication has 37 references indexed in Scilit:
- A Novel Nucleoprotein Complex at a Replication OriginScience, 1990
- Structure and expression of the ompB operon, the regulatory locus for the outer membrane porin regulon in Salmonella typhimurium LT-2Journal of Molecular Biology, 1988
- Sequence comparison of single-stranded DNA binding proteins and its structural implicationsJournal of Molecular Biology, 1987
- SINGLE-STRANDED DNA BINDING PROTEINS REQUIRED FOR DNA REPLICATIONAnnual Review of Biochemistry, 1986
- Linear DNA replication: inverted terminal repeats of five closely related Escherichia coli bacteriophagesGene, 1986
- An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoterCell, 1985
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replicationGene, 1983
- Comparison of the Lipid-containing Bacteriophages PRD1, PR3, PR4, PR5 and L17Journal of General Virology, 1981
- Characterization by electron microscopy of the complex formed between T4 bacteriophage gene 32-protein and DNAJournal of Molecular Biology, 1972