Vitamin E: a sensor and an information transducer of the cell oxidation state
- 1 December 1995
- journal article
- research article
- Published by Elsevier in The American Journal of Clinical Nutrition
- Vol. 62 (6) , 1337S-1346S
- https://doi.org/10.1093/ajcn/62.6.1337s
Abstract
We studied the effects of RRR-alpha-tocopherol and RRR-beta-tocopherol in smooth muscle cells from rat (line A7r5) and human aortas. RRR-alpha-Tocopherol, but not RRR-beta-tocopherol, inhibited smooth muscle cell proliferation in a dose-dependent manner at concentrations in the range from 10 to 50 mumol/L. RRR-beta-Tocopherol added simultaneously with RRR-alpha-tocopherol prevented growth inhibition. The earliest event brought about by RRR-alpha-tocopherol in the signal transduction cascade controlling receptor-mediated cell growth was the activation of the transcription factor AP-1. RRR-beta-tocopherol alone was without effect but in combination with RRR-alpha-tocopherol prevented the AP-1 activating effect of the latter. Protein kinase C was inhibited by RRR-alpha-tocopherol and not by RRR-beta-tocopherol, which also in this case prevented the effect of RRR-alpha-tocopherol. Calyculin A, a protein phosphatase inhibitor, prevented the effect of RRR-alpha-tocopherol on protein kinase C. The data can be rationalized by a model in which a tocopherol-binding protein discriminates between RRR-alpha-tocopherol and RRR-beta-tocopherol and initiates a cascade of events at the level of cell signal transduction that leads to the inhibition of cell proliferation.Keywords
This publication has 65 references indexed in Scilit:
- Regulation of the transcription factors NF-κB and AP-1 by redox changesChemico-Biological Interactions, 1994
- Inhibition of NF-κB Activation by Vitamin E DerivativesBiochemical and Biophysical Research Communications, 1993
- The pathogenesis of atherosclerosis: a perspective for the 1990sNature, 1993
- Comparative antioxidant activity of tocotrienols and other natural lipid-soluble antioxidants in a homogeneous system, and in rat and human lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1993
- Purification and partial characterisation of and ?-tocopherol-binding protein from rabbit heart cytosolMolecular and Cellular Biochemistry, 1993
- Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase CBiochemical and Biophysical Research Communications, 1992
- Vitamin E inhibits protein oxidation in skeletal muscle of resting and exercised ratsBiochemical and Biophysical Research Communications, 1992
- Identification of three types of PDGF-A chain gene transcripts in rabbit vascular smooth muscle and their regulated expression during development and by angiotensin IIBiochemical and Biophysical Research Communications, 1992
- Calyculin A and okadaic acid: Inhibitors of protein phosphatase activityBiochemical and Biophysical Research Communications, 1989
- Lack of Tocopherol in Peripheral Nerves of Vitamin E-Deficient Patients with Peripheral NeuropathyNew England Journal of Medicine, 1987