Gas Chromatographic Assay of Epoxide Hydrase Activity with 3-Methylcholanthrene-11,12-Oxide
- 7 September 1973
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 181 (4103) , 951-952
- https://doi.org/10.1126/science.181.4103.951
Abstract
Epoxide hydrase has been measured in rat tissue with 3-methylcholanthrene-11,12-oxide as substrate; diol formation was assayed by gas chromatographic separation of the trimethylsilylated derivative of trans-11,12-dlhydro-11,- 12-dihydroxy-3-methylcholanthrene from the corresponding derivative of the 11 (or 12)-hydroxy-3-methylcholanthrene on 3 percent OV-17, which is formed from the 11,12-oxide during the derivatization. The polycyclic hydrocarbons were extracted initially from the incubation mixture with ethyl acetate. The assay is simple, inexpensive, and sensitive.Keywords
This publication has 12 references indexed in Scilit:
- Isolation and characterization of an active DNA-binding metabolite of benzo(a)pyrene from hamster liver microsomal incubation systemsBiochemical and Biophysical Research Communications, 1972
- Hepatic epoxide hydrase. Structure-activity relations for substrates and inhibitorsBiochemistry, 1971
- Epoxy derivatives of aromatic polycyclic hydrocarbons. The preparation of benz[a]anthracene 8,9-oxide and 10,11-dihydrobenz[a]anthracene 8,9-oxide and their metabolism by rat liver preparationsBiochemical Journal, 1971
- Epoxides as microsomal metabolites of polycyclic hydrocarbonsFEBS Letters, 1971
- An epoxide is an intermediate in the microsomal metabolism of the chemical carcinogen, dibenz(a,h)anthraceneBiochemical and Biophysical Research Communications, 1971
- Substrate specificity of hepatic epoxide hydrase in microsomes and in a purified preparation: Evidence for homologous enzymesArchives of Biochemistry and Biophysics, 1971
- A radiometric assay for hepatic epoxide hydrase activity with [7-3H] styrene oxideBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Role of the arene oxide-oxepin system in the metabolism of aromatic substrates. IV. Stereochemical considerations of dihydrodiol formation and dehyrogenationJournal of the American Chemical Society, 1970
- The conversion of phenanthrene 9, 10-oxide and dibenz[a, h]anthracene 5, 6-oxide into dihydrodiols by a rat-liver microsomal enzymeBiochemical Pharmacology, 1970
- AN ENZYME CATALYSING THE CONJUGATION OF EPOXIDES WITH GLUTATHIONEBiochemical Journal, 1965