CITRATE AND THE CONVERSION OF CARBOHYDATE INTO FAT. ACTIVITIES OF CITRATE-CLEAVAGE ENZYME AND ACETATE THIOKINASE IN LIVERS OF NORMAL AND DIABETIC RATS

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Abstract
The activity of citrate-cleavage enzyme declines in alloxan-diabetes. The administration of insulin elevates the activity of the enzyme in livers of normal and diabetic animals. Diets high in glucose or fructose elevate the activity of citrate-cleavage enzyme in normal animals, whereas only the diet high in fructose does so in diabetic animals. These observations parallel the effects of insulin, glucose and fructose on fatty acid synthesis in normal and diabetic animals. The effect of fructose is more rapid and larger than the effect of glucose. With 1 exception acetate thiokinase shows similar changes at a lower level of activity. The results indicate that insulin acts by increasing glucose utilization, and not by exerting a direct effect on citrate-cleavage enzyme or acetate thiokinase.