Glucose transport in Streptococcus mutans: preparation of cytoplasmic membranes and characteristics of phosphotransferase activity.

  • 1 March 1975
    • journal article
    • Vol. 54  (2) , 330-8
Abstract
Cytoplasmic membranes from Streptococcus mutans were used to study phosphoenolpyruvate (PEP)-dependent glucose transport. The Km values for glucose and PEP were 0.3 and 0.4 mM. The system was sensitive to sulfhydryl reagents and detergents. The nonmetabolizable analogue 2-D-deoxyglucose was readily phosphorylated while alpha-methyl-glucose and 3-0-methylglucose were inefficiently derivatized. The fermentable sugars fructose, galactose, mannitol, and sorbitol were phosphorylated at reduced rates.

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