Comparison of PCR and antigen detection methods for diagnosis ofEntamoeba histolyticainfection
Open Access
- 24 November 2004
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 57 (12) , 1264-1266
- https://doi.org/10.1136/jcp.2004.017822
Abstract
Aims:To assess different laboratory methods for the identification ofEntamoeba histolyticain clinical samples.Methods:Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR were compared using 101 stool and pus samples.Results:Fifteen of the 101 samples were positive forE histolyticaby one or more method. There were discrepancies between the results in five of these 15 samples when the assays were compared.Conclusions:All three methods performed adequately, so that the choice of assay will depend on each individual laboratory’s budget and projected turnaround time.Keywords
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