ISOLATION AND PRELIMINARY CHARACTERIZATION OF MYCOPLASMAVIRUS 20-P

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 60  (2) , 116-120
Abstract
A mycoplasmavirus, MV 20-P, was isolated from the fastidious, slow-growing human mycoplasma 20-P, previously identified by the growth inhibition test as Mycoplasma hominis type 2. Its broth culture was ultracentrifuged into 35% saccharose, the pellet was resuspended in buffer and the suspension was dropped onto lawns of the virus indicator Acholeplasma laidlawii BCL-13. The virus was washed off the plaque areas by buffer. MV 20-P appeared in EM as spherical, enveloped particles .apprx. 80 nm in diameter. It was sensitive to lipid solvents. MV 20-P appears to be an MVL2 type mycoplasmavirus and may possibly be the first of human origin.