Bacteria of the genus Asaia stably associate with Anopheles stephensi , an Asian malarial mosquito vector
Top Cited Papers
- 22 May 2007
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 104 (21) , 9047-9051
- https://doi.org/10.1073/pnas.0610451104
Abstract
Here, we show that an α-proteobacterium of the genus Asaia is stably associated with larvae and adults of Anopheles stephensi, an important mosquito vector of Plasmodium vivax, a main malaria agent in Asia. Asaia bacteria dominate mosquito-associated microbiota, as shown by 16S rRNA gene abundance, quantitative PCR, transmission electron microscopy and in situ-hybridization of 16S rRNA genes. In adult mosquitoes, Asaia sp. is present in high population density in the female gut and in the male reproductive tract. Asaia sp. from An. stephensi has been cultured in cell-free media and then transformed with foreign DNA. A green fluorescent protein-tagged Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission. The larval gut and the male reproductive system were also colonized by the transformed Asaia sp. strain. As an efficient inducible colonizer of mosquitoes that transmit Plasmodium sp., Asaia sp. may be a candidate for malaria control.Keywords
This publication has 42 references indexed in Scilit:
- Advantage of Using Inosine at the 3′ Termini of 16S rRNA Gene Universal Primers for the Study of Microbial DiversityApplied and Environmental Microbiology, 2006
- Sexual acquisition of beneficial symbionts in aphidsProceedings of the National Academy of Sciences, 2006
- First Report of Bacteremia by Asaia bogorensis , in a Patient with a History of Intravenous-Drug AbuseJournal of Clinical Microbiology, 2006
- AgDscam, a Hypervariable Immunoglobulin Domain-Containing Receptor of the Anopheles gambiae Innate Immune SystemPLoS Biology, 2006
- Production of the antimalarial drug precursor artemisinic acid in engineered yeastNature, 2006
- A Novel Bacteroidetes Symbiont Is Localized in Scaphoideus titanus , the Insect Vector of Flavescence Dorée in Vitis viniferaApplied and Environmental Microbiology, 2006
- Using bacteria to express and display anti-parasite molecules in mosquitoes: current and future strategiesInsect Biochemistry and Molecular Biology, 2005
- Sequence and Expression Analysis of the ompA Gene of Rickettsia peacockii , an Endosymbiont of the Rocky Mountain Wood Tick, Dermacentor andersoniApplied and Environmental Microbiology, 2004
- Asaia sp., an Unusual Spoilage Organism of Fruit-Flavored Bottled WaterApplied and Environmental Microbiology, 2002
- Bacterial Symbionts of the Triatominae and Their Potential Use in Control of Chagas Disease TransmissionAnnual Review of Entomology, 2002