Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 68 (1) , 23-28
- https://doi.org/10.1007/bf00498497
Abstract
Several fluorescent maleimide compounds were evaluated as possible substitutes for N-(4-aminophenyl)maleimide in the histochemical procedures developed by Sippel (1973, 1978a, b, 1980) for the demonstration of sulfhydryl and disulfide groups. The brightest and most selective fluorescence was obtained by using N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), although both eosin-5-maleimide and fluorescein-5-maleimide could also be used if adequate control preparations were made.This publication has 7 references indexed in Scilit:
- The histochemistry of thiols and disulphides. IV. Protective fixation by organomercurial-formalin mixturesJournal of Molecular Histology, 1980
- The histochemical distribution of protein bound sulfhydryl groups in human epidermis by the new staining method.Journal of Histochemistry & Cytochemistry, 1979
- The histochemistry of thiols and disulphides. III. Staining patterns in rat tissuesJournal of Molecular Histology, 1978
- The histochemistry of thiols and disulphides. II. Methodology of differential stainingJournal of Molecular Histology, 1978
- N-(7-Dimethylamino-4-methyl-coumarinyl) maleimides (DACM) : Novel Fluorescent Thiol ReagentsCHEMICAL & PHARMACEUTICAL BULLETIN, 1975
- the histochemistry of thiols and disulphides. I. The use of N-(4-aminophenyl)maleimide for demonstrating thiol groupsJournal of Molecular Histology, 1973
- Fluorescent thiol reagentsAnalytical Biochemistry, 1972