Synthesis and Metabolism of 5β-[11, 12-3H]Cholestane-3α, 7α-Diol
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 83 (2) , 379-383
- https://doi.org/10.1093/oxfordjournals.jbchem.a131924
Abstract
5β-β11, 12-3H]Cholestane-3α, 7α-diol was synthesized as follows. 5β Cholestane-3α, 7α, 12α-triol 3, 7-diacetate was treated with phosphorus oxychloride in pyridine solution and then the product, 5β-cholest-11-ene-3α, 7α-diol diacetate, was hydrogenated in acetic acid solution using platinum oxide as a catalyst under an atmosphere of tritium gas. 5β-[11, 12-3H]Cholestane-3α, 7α-diol thus obtained was readily hydroxylated at C-26 by mitochondria in the presence of isocitric acid, magnesium chloride and potassium cyanide.This publication has 6 references indexed in Scilit:
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