Recycling HPLC ofp-Bromophenacyl Esters of Saturated C35–56Fatty Acids fromMycobacterium TuberculosisH37Ra on a Silica Column
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 2 (6) , 861-873
- https://doi.org/10.1080/01483917908060109
Abstract
The p-bromophenacyl esters of saturated C35–56 fatty acids from Mycobacterium tuberculosis H37Ra were separated according to structural classes on a silica column by high performance liquid chromatography (HPLC). The sample was cycled five times during HPLC. Highly purified C35–38 esters were obtained by this method. Further HPLC fractionation on a reverse-phase column (C18-bonded silica) gave complete separation of most of the remaining fractions. By combining mass spectrometry with HPLC separations, many of the fatty acid esters were tentatively identified.This publication has 7 references indexed in Scilit:
- Characterization of the purified components of a new homologous series of alpha-mycolic acids from Mycobacterium tuberculosis H37RaJournal of Biological Chemistry, 1978
- Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatographyAnalytical Chemistry, 1975
- The mycobacterial cell wallPublished by Walter de Gruyter GmbH ,1971
- Chemical analyses of mycobacterial cell wallsBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- Fatty acids, part 16. Thin layer and gas—liquid chromatographic properties of the cis and trans methyl octadecenoates and of some acetylenic estersChemistry and Physics of Lipids, 1967