Capillary Electrophoresis STR Analysis: Comparison to Gel-Based Systems
- 1 January 1998
- journal article
- research article
- Published by ASTM International in Journal of Forensic Sciences
- Vol. 43 (1) , 164-170
- https://doi.org/10.1520/jfs16104j
Abstract
Capillary electrophoresis is a relatively new technology for the forensic analysis of STRs, and may be an excellent alternative to traditional gel-based methods. Eighty samples, obtained from six different laboratories which had previously analyzed the samples using a variety of gel-based methods, were reanalyzed by CE. It was determined that CE could obtain the same allelic designation as that reported by the comparison laboratory. Seven different loci employing two separate multiplex amplifications were used for the comparison study. One basepair resolution and average standard deviations within the range of 0.075 to 0.117 basepairs were observed.Keywords
This publication has 13 references indexed in Scilit:
- Multiplex Sets for the Amplification of Polymorphic Short Tandem Repeat Loci — Silver Stain and Fluorescence DetectionBioTechniques, 1996
- Further validation of a multiplex STR system for use in routine forensic identity testingForensic Science International, 1996
- PCR Amplification of Animal DNA with Human X-Y Amelogenin Primers Used in Gender DeterminationJournal of Forensic Sciences, 1995
- Application of dual internal standards for precise sizing of polymerase chain reaction products using capillary electrophoresisElectrophoresis, 1995
- Three tetranucleotide polymorphisms for loci: D3S1352; D3S1358; D3S1359Human Molecular Genetics, 1993
- Automated DNA profiling employing multiplex amplification of short tandem repeat loci.Genome Research, 1993
- Tetranucleotide repeat polymorphism at the human alpha fibrinogen locus (FGA)Human Molecular Genetics, 1992
- A further tetranucleotide repeat polymorphism in the vWF geneHuman Molecular Genetics, 1992
- Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locusHuman Molecular Genetics, 1992
- A human X-Y homologous region encodes “amelogenin”Genomics, 1991