Phosphoenolpyruvate Carboxykinase Activity in Grape Berries
Open Access
- 1 July 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 56 (1) , 67-71
- https://doi.org/10.1104/pp.56.1.67
Abstract
Phosphoenolpyruvate (PEP) carboxykinase activity was found in crude extracts of `Pinot noir' grape berries. The enzyme required ATP, Mn2+ plus Mg2+, a pH of 6.6, and a temperature of 40 C for maximum activity. The range in concentration of oxaloacetic acid needed for maximum phosphoenolpyruvate carboxykinase activity was 5 to 10 mm, and the Km for HCO3- in the exchange of 14CO2 into oxaloacetic acid was 26.8 mm.Keywords
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