Abstract
Trigonellin and stachydrin were identified in the tissues by their crystal formation with potassium bismuth iodide. Quantitative determinations also were made. Stachydrin plants examined were Stachys palustris, S. recta and Galeopsis ochroleuca; trigonellin plants were Trigonella joenum graecum, T. coerulea and Dahlia variabilis. In all the plants there was a relatively greater amount of the particular betaine in the flowers and roots than in other organs. Etiolated seedlings of Trigonella joenum graecum, compared with green seedlings, contained more trigonellin from the 6th to 15th day, but less later. On supplying proline, ornithine or glutamic acid to the plants the betaines were increased; glycocoll gave no increase. The best method was by injection into the hollow sterna of the plants. Control plants were injected with water. The greatest yield was obtained by the injection of hexamethylenetetramine together with proline into the stems.

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