Different exon-intron organization at the 5' part of a mouse class I gene is used to generate a novel H-2Kd-related mRNA.

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Abstract
A c[complementary]DNA library constructed from liver mRNA of DBA/2 (H-2d) mice was screened with H-2-specific probes. Nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene with an unexpected exon-intron organization. Nucleotide sequence comparisons suggest that 2 distinct mRNA are prodced from a single H-2Kd gene by a mechanism involving the use of alternative splicing sites in its 5'' region. Clone pH-2d-24 carries an open reading frame encoding a thus-far-undescribed polypeptide product identical to an H-2Kd-molecule, except for the NH2-terminal half of the 1st domain.