INVITRO HISTAMINE-RELEASE FROM HUMAN BASOPHILS TRIGGERED BY A PURIFIED ALLERGEN FROM DERMATOPHAGOIDES-FARINAE - BIMODAL ASPECT OF THE DOSE-RESPONSE CURVE

  • 1 January 1982
    • journal article
    • research article
    • Vol. D133  (1) , 87-94
Abstract
Histamine release experiments were performed over a 106-fold range of allergen concentrations on basophils of patients sensitive to D. farinae. Two extracts of D. farinae were used: a partially purified one (Df 80d) containing several allergens and a highly purified component (Ag11). These histamine release patterns obtained with the complex allergen mixture Df 80d gave broad histamine release curves presenting an initial increase, followed by a decrease or a plateau, with occasionally a small dip. The Ag11-induced histamine release curves presented 2 peaks (bimodal pattern), separated by a relatively pronounced inhibition dip. No nonspecific histamine releasing activity was observed with the basophils of 2 controls. The possible theoretical models for the bimodal histamine release patterns are discussed.