Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
Open Access
- 1 January 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (1) , 459-463
- https://doi.org/10.1073/pnas.78.1.459
Abstract
The excision of the Kanamycin resistant determinant Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. The frequency of excision depended on the site of Tn5 insertion, excision occurred efficiently in RecA- cells, excision did not require a Tn5-encoded transposition function and efficient excision required the inverted repeats of Tn5. Excision of Tn5 may be similar to the formation of spontaneous deletions and may occur by slippage during DNA synthesis.Keywords
This publication has 28 references indexed in Scilit:
- Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance elementMolecular Genetics and Genomics, 1979
- Genetic studies of the lac repressorJournal of Molecular Biology, 1978
- Transposition of a DNA sequence determining kanamycin resistance into the single-stranded genome of bacteriophage fdMolecular Genetics and Genomics, 1978
- A genetic analysis of the att-int-xis region of coliphage lambdaJournal of Molecular Biology, 1977
- Transposition of R factor genes to bacteriophage lambda.Proceedings of the National Academy of Sciences, 1975
- Reversal of mutator phage Mu integrationJournal of Molecular Biology, 1975
- Packaging of prophage and host DNA by coliphage λNature, 1975
- Electron microscope study of the structures of λdv DNAsJournal of Molecular Biology, 1974
- Strand Displacement during Deoxyribonucleic Acid Synthesis at Single Strand BreaksJournal of Biological Chemistry, 1971
- Enzymic synthesis of deoxyribonucleic acidJournal of Molecular Biology, 1964