Colorimetric Assay of Caffeine in Crude Drugs and in Pharmaceutical Preparations

Abstract
The well known Murexide reaction for purine bases was modified and optimized for the quantification of caffeine in crude drugs, refreshing drinks [coffee, tea and cola-type drinks] and pharmaceuticals. The method was also applied for the detection and assay of caffeine in mixtures of illegal opiate samples [heroin and opium]. It was also applied to assay theobromine and theophylline alongside with caffeine in their natural and synthetic mixtures after a simple separating procedure depending on their different bascities.

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