Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR
- 1 January 1995
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 33 (1) , 248-50
- https://doi.org/10.1128/jcm.33.1.248-250.1995
Abstract
Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.Keywords
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